| Title | A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients. |
| Publication Type | Journal Article |
| Year of Publication | 2021 |
| Authors | Morciano G, Pedriali G, Bonora M, Pavasini R, Mikus E, Calvi S, Bovolenta M, Lebiedzinska-Arciszewska M, Pinotti M, Albertini A, Wieckowski MR, Giorgi C, Ferrari R, Galluzzi L, Campo G, Pinton P |
| Journal | Cell Rep |
| Volume | 35 |
| Issue | 2 |
| Pagination | 108983 |
| Date Published | 2021 Apr 13 |
| ISSN | 2211-1247 |
| Keywords | Aged, Animals, Base Sequence, Calcium Channels, Exons, Female, Gene Expression, Humans, Hypoxia, Introns, Male, Middle Aged, Mitochondria, Mitochondrial Permeability Transition Pore, Mitochondrial Proton-Translocating ATPases, Mutation, Myocytes, Cardiac, Oxygen, Prospective Studies, Reperfusion Injury, ST Elevation Myocardial Infarction |
| Abstract | Preclinical models of ischemia/reperfusion injury (RI) demonstrate the deleterious effects of permeability transition pore complex (PTPC) opening in the first minutes upon revascularization of the occluded vessel. The ATP synthase c subunit (Csub) influences PTPC activity in cells, thus impacting tissue injury. A conserved glycine-rich domain in Csub is classified as critical because, when mutated, it modifies ATP synthase properties, protein interaction with the mitochondrial calcium (Ca) uniporter complex, and the conductance of the PTPC. Here, we document the role of a naturally occurring mutation in the Csub-encoding ATP5G1 gene at the G87 position found in two ST-segment elevation myocardial infarction (STEMI) patients and how PTPC opening is related to RI in patients affected by the same disease. We report a link between the expression of ATP5G1 and the response to hypoxia/reoxygenation of human cardiomyocytes, which worsen when compared to those expressing the wild-type protein, and a positive correlation between PTPC and RI. |
| DOI | 10.1016/j.celrep.2021.108983 |
| Alternate Journal | Cell Rep |
| PubMed ID | 33852870 |