At the Englander Institute for Precision Medicine (EIPM), we strive to be at the forefront of translational research. We’ve recently paired the Helios™, mass cytometry by time-of-flight (CyTOF) instrument with the Hyperion imaging module. The system is one of 30 of its kind in use, and the only one in the NYC area.
With this advanced technology for single-cell analysis, we are providing investigators with a powerful, affordable and actionable tool for exploring complex biological processes.
“The CyTOF and Hyperion will enable exciting new discoveries regarding the structure of disease tissue at the single cell level, especially the role of immune cells.”
– EIPM Director Olivier Elemento, Ph.D.
Graphic courtesy: Fluidigm
Technology Overview:
CyTOF aids in multiplexing and deep profiling of single cells, and is expected to have significant clinical applications. With this Inductively Coupled Plasma (ICP) – Time of Flight-based technology, one can profile 40 markers at a time on a single cell and learn more about the functional and phenotypic relevance of the cell state in a tissue context.
The Hyperion™ Imaging System is a mass cytometry-based high-resolution laser ablation system that allows for the highly multiplexed imaging with up to 135 channels available. The system is designed to detect metal-tagged antibodies bound to the cell surface and intracellular proteins in tissue sections using immunohistochemical methods. The system allows for high-resolution cellular profiling in spatial proximity, enabling detection of disease cells and immune cell populations within the context of the tissue structure.
Imaging Mass Cytometry employs laser ablation of tissue sections stained with a leap in dimensionality of 40 markers simultaneously, generating millions of pixels with a resolution comparable to light microscopy but with high content of mass cytometry. This technology unveils the interaction between cell types in their tissue context and throws more light into the characterization of functional and signaling state of the cells.
| Tissues analyzed | FFPE, frozen tissues, biopsies |
| Targets analyzed | Proteins and RNAs |
| Tissue thickness (full ablation) | ≤7 µm |
| Addressable sample size | ≥16 mm x 55 mm |
| Scan area | ≥1 mm2/ 4 Hr (@100Hz) |
| Ablation spot size | ≤20 % |
| Optimal operating temperature | 21 ± 1 °C |
Graphic courtesy: Paymaneh D Malihi et al 2018 Converg. Sci. Phys. Oncol. 4 015003
| Workflow | Applications |
| Frame relevant research goal | Immuno-phenotyping |
| Identify markers that augment research goal | Functional and Signaling state of a cell in tissue context |
| Design the panel | Cytokine/Chemokine expression |
| Antibody validation | Normal vs. Diseased state comparison to identify any clinically significant cell transformation |
| Antibody Titration | Proliferation analyses |
| Staining and scanning on Hyperion | Apoptosis and Necrosis profiling |
| Analysis of the data (cell segmentation, ViSNE plot, etc.) |
Services & Fees
| Pricing Category | Internal / External Academic Institutions | Industry |
| Sample Acquisition (Helios) | $100 per hour | $160 per hour |
| Tissue Ablation (Hyperion) | $120 per hour | $190 per hour |
| Metal-tagged antibody | $3-$5 per ul | $3-$5 per ul |
| Metal-tagged antibody (CST vendor) | $7 -$10 per ul | $7 -$10 per ul
|
* Stock concentration of the antibodies: 0.5 mg/ml.
* CyTOF ready antibodies from Cell Signaling Technologies is comparatively more expensive than other vendors, which is reflected in our pricing.
CyTOF Sample Run Request Form
WCM and NewYork-Presbyterian clinicians must submit a CyTOF Project Request before submitting samples. For projects already submitted, you may submit a CyTOF Service Request. This technology will be available to researchers outside of our network by contacting Ms. Hiranmayi Ravichandran: hir4001@med.cornell.edu
Click for Reagents and Antibodies list
Links to Resources and Publications:
- Mass Cytometry Publications
- Trending Publications
- The underlying principles of this technology and emerging applications are explained
- Recent Publications
- For more information and assistance
Links to Protocols:
- Imaging Mass Cytometry Staining Protocol for FFPE Sections
- Maxpar Cell Surface Staining with Fresh Fix
- Maxpar Nuclear Antigen Staining with Fresh Fix
- Maxpar Phosphoprotein Staining with Fresh Fix
- Maxpar Cytoplasmic/Secreted Antigen Staining with Fresh Fix
*Please email hir4001@med.cornell.edu for up-to date protocols
The acquisition of EIPM’s CyTOF technology was made possible through a generous donation from Mr. Igor Tulchinsky and the WorldQuant, LLC Initiative for Quantitative Prediction.
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