Englander Institute for Precision Medicine

Proteogenomic analysis of pediatric and AYA high-grade glioma reveals age-dependent biology, female-male differences, and kinase targets.

TitleProteogenomic analysis of pediatric and AYA high-grade glioma reveals age-dependent biology, female-male differences, and kinase targets.
Publication TypeJournal Article
Year of Publication2026
AuthorsTignor NL, Koptyra M, Chowdhury S, Petralia F, Gritsenko MA, Ma W, Zhu Y, Marino GB, Song X, Migliozzi S, Whiteaker JR, Rykunov D, Hu Y, Hosseini N, Rokita JLynne, Leprevost Fda Veiga, Rathi KS, Chen L, Wang Y-T, Weitz KK, Chu RK, Moore RJ, Krek A, Fu W, Wang X, Deng EZ, Tsai C-F, Sagendorf T, Petyuk VA, Shi T, Fillmore TL, Zhao R, Monroe ME, Dannappel MV, Daniel P, Zhao L, Ivey RG, Voytovich UJ, Yaron-Barir TM, Huntsman EM, Johnson JL, Abedin N, Li Y-C, Santi M, Dupal D, Lilly J, Kraya A, Dybas JM, Zhang B, Zhong C, Brown MA, Phul S, Wafula E, Farrel A, Geng Z, Corbett RJ, Naqvi AS, Miller DP, Mason J, Patton TS, McGrory S, Robins S, Heath A, Sullivan C, Coleman N, Morgan A, Garofano L, Reva B, Schadt EE, Smith RD, Mesri M, Robles AI, Cantley LC, Ding L, Rodland KD, Zhang B, Nesvizhskii AI, Iavarone A, Cieslik M, Ippolito JE, Storm PB, Rubin JB, Firestein R, Ma'ayan A, Zhang H, Paulovich AG, Liu T, Resnick A, Rood BR, Wang P
Corporate AuthorsPhiladelphia Coalition for a Cure, Children’s Brain Tumor Network, Clinical Proteomic Tumor Analysis Consortium
JournalCell Rep Med
Pagination103024
Date Published2026 Sep 17
ISSN2666-3791
Abstract

High-grade gliomas (HGGs) in children and adolescents and young adults (AYA) exhibit distinct biology across the neurodevelopmental spectrum. To dissect tumor-intrinsic molecular characteristics independent of developmental variation, we perform comprehensive proteogenomic analyses of tumors from 112 HGG patients aged 0-40 years. Our multi-omics analysis identifies two AYA subgroups-adolescents (aged 15-26 years) and young adults (aged 26-40 years)-with distinct molecular profiles and survival outcomes. Tumor-normal comparisons and survival modeling highlight roles of oxidative phosphorylation and neuronal system biology in glioma progression. Causal network analysis and cell line studies provide a rationale for personalized therapies targeting candidate kinases, such as CDK8. Survival modeling, clustering, and immune-landscape analyses identify proteins, post-translational modifications, and immune signatures linked to outcomes and reveal clinically relevant differences between male and female patients.

DOI10.1016/j.xcrm.2026.103024
Alternate JournalCell Rep Med
PubMed ID42753723

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